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KMID : 0620919980300040199
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Experimental & Molecular Medicine
1998 Volume.30 No. 4 p.199 ~ p.204
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Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells
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R K Saxena/Niti Puri
R K Saxena
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Abstract
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Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.
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KEYWORD
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B cells, proliferation, cytokines, LPS, growth factor,
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